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Its a bit like trying to pump up a tyre - it's quite easy when there isn't air in the tyre but gets more difficult as the tyre pumps up with air

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The 100 mg/kg bw/day used in the prevention study came about after an evaluation of the intervention study results and with the knowledge that Cremer, et al, [35], used up to 180 mg/kg bw/day for 24 months led to no observable effects of ALA on histology or pathology

doi: 10.1006/abbi.1996.0146