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doi: 10.1002/ctm2.334 63 ShengQLiFChenGLiJLiJWangYet al

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For the confocal microscopy, PSCs were seeded onto 8-well chambered coverglass slides (Lab-TEK, NalgeNunc) at 5 10 3 cells per well, overnight, and incubated in DMEM (without serum) containing Au@PP/Cy5-siRNA or Au@PP/RA/Cy5-siRNA (4.2 M ATRA, 50 nM siRNA) at pH 6.5 or 7.4, respectively, for 2 h