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Hoechst+ and mNeonGreen+ cells were counted by the Gen5 software (Biotek, Winooski, VT, USA), and infection rates were calculated using the ratio of mNeonGreen+/Hoechst+ cells

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ATCC CCL-81) were used for the titration

One possibility is that diminished expression of Slc7a11 in Nrf2-null mice limits the supply of intracellular cystine, which is reduced to cysteine for protein synthesis [164], and that lower levels of cysteine may compromise the correct folding of membrane proteins and secretory proteins within the ER Another possibility is that the diminished availability of GSH, or a relative inability to reduce GSSG, compromises the ability of cells to fold proteins into their appropriate native tertiary structures