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In animal studies, AM404 is a potent agonist at the TRPV1 receptor that mediates pro-inflammatory and painful stimuli [49,52]

This NADP + -dependent dimerization/monomerization modulates FRET between the homologous fluorescent proteins (homoFRET) resulting in changes in steady-state fluorescence anisotropy that can then be used to assess the redox state of NADP + /NADPH

Besides being the sole antioxidant enzyme able to repair lipid peroxides, GPX4 is a moonlighting protein that also regulate eicosanoid biosynthesis ( Hydroperoxides relevant to inflammation are those formed from arachidonic acid (AA) by stereoselective lipoxygenases

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