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PLP is a cofactor for the synthesis of several neurotransmitters including serotonin, the catecholamines dopamine, norepinephrine, and epinephrine [critical enzyme is aromatic L-amino acid decarboxylase (AADC) which is encoded by the DDC gene and is more commonly referred to as DOPA decarboxylase], and -aminobutyric acid, GABA (critical enzyme is glutamic acid decarboxylase, GAD)

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Since the melatonin exhibits its action through the involvement of various molecular pathways, the most described pathways involve activation of specific membrane receptors namely ML1 i.e., high affinity and low affinity i.e., ML2 sites (Dubocovich 1995

The doses were administered in a randomized sequence with a 1-week wash-out period between each administration