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Description
Proteins were visualized using the Supersignal West Pico Chemiluminescent Substrate system (Pierce, Fenton, MO) and molecular weights determined by comparison to protein ladders (BenchMark, Invitrogen
Bott et al , 2015
Refrigerate all reconstituted peptides at 2-8 degrees Celsius
of alpha-fetoprotein (AFP) with femtomolar accuracy in human serum
