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Dilute cells to a density of 7.5 10 5 cells/mL in RPMI 1640 cell culture media (2 mM L-glutamine, 10% FBS)

Given the rich lipid content of the nervous system, hemoglobin-derived iron works with lipid peroxidation to induce ferroptosis, which eventually leads to neuronal mitochondrial damage, edema, and apoptosis of neurons and astrocytes

Archaeological evidence suggests that its usage dates to 10,000 years ago by ancient civilizations in regions such as Mesopotamia, Egypt, China, and India [1]
