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A SYBR Premix Ex Taq II (Tli RNaseH Plus) Kit was used to perform qRT-PCR in technical triplicate on a StepOnePlus Real-time PCR System (Applied Biosystems, USA) following the manufacturers instructions

This event, in an inflammatory environment, triggers the breakdown of immune tolerance and activates autoreactive CD4 + T cells that have escaped central and peripheral control mechanisms

Environ Pollut 266(2):115297 Fernandes KE, Frost EA, Remnant EJ, Schell KR, Cokcetin NN, Carter DA (2022) The role of honey in the ecology of the hive: nutrition, detoxification, longevity, and protection against hive pathogens

Genomic studies have associated chromosomal abnormalities and pathogenic variants with early embryonic developmental failure, while epigenomic profiling has highlighted aberrant methylation patterns and imprinting disturbances