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Next, we used the pro-inflammatory cytokines tumor necrosis factor- (TNF-) and interleukin-6 (IL-6) as indicators to evaluate the anti-inflammatory response

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doi:10.3390/antiox10091473 Mignone LE, Wu T, Horowitz M, Rayner CK

Afterward, the supernatant was collected and diluted to a final concentration of 53% methanol with LCMS grade water
