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CACT acetylation mechanism is in line with a dynamic post-translational protein control that could undergo an on/off switch induced by deacetylation/acetylation linked to the availability of acetyl-CoA 57

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A suboptimal intake of the essential amino acids increases the body's need for the non-essential amino acids either through diet or supplementation

Citrulline is a non-essential amino acid that is an important intermediate in the urea cycle, functioning along with other amino acids to rid the body of ammonia, a byproduct of protein metabolism