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The second portion was frozen in liquid nitrogen and stored at 80 C for RNA extraction

Study strengths and limitations A key strength of this study is the replication of findings in an independent cohort ( enable cohort), supporting the robustness of our results

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This review consolidates existing empirical evidence regarding the collective contributions of CIT, leucine, and physical exercise in fostering healthy aging, while also delineating prospective research avenues for the formulation of personalized nutritional and physical strategies aimed at enhancing both muscular and vascular well-being in the elderly population