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YUMM3.3SelO cells stably expressing SelO, SelO D338A, or SelO V334A were lysed in 25 mM Tris pH 7.5, 150 mM NaCl, 1% Triton-100, 1 mM EDTA, 1 mM EGTA, 1x PIC, 1 mM DTT
