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Subsequent study on rabbit nonunion critical size defect model revealed that tanshinol bone-targeted liposomes incorporated with collagen sponge can significantly increase the expression of type II collagen, Runx2, VEGFA and osteocalcin in vivo , and stimulate nonunion healing by regulating histone deacetylase 3 (HDAC3) -mediated endochondral ossification (Zhou et al., 2020) (Table 7)
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