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Co-cultures were stimulated with 50 ng/mL phorbol-12-myristate-13-acetate (PMA) and 750 ng/mL ionomycin (both Sigma-Aldrich) for 4h in the presence of 2 M monensin (Biolegend) at 37C and 5% CO 2/ /95% humidified air
This iterative approach prevents both under-treatment and unnecessary over-treatment as conditions evolve
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doi: 10.1016/j.cell.2020.05.015
