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Methods Cell lines and culture conditions Murine hepatocyte cell line AML12 (Alpha Mouse Liver 12, ATCC CRL-2254) and human embryonic kidney (HEK) 293 T cell line (ATCC CRL-3216) were originally obtained from American Tissue Culture Collection Biobank (Manassas, VA, USA) and were incubated at 37C with 5% CO2

Existing drugs such as GC therapy, which upregulates CD163, anti-diabetic drug, pioglitazone, which upregulates CD36, and a kinase inhibitor, imatinib, which upregulates SRBI, may be evaluated for potential in regulating the clearance of hemolysis products at the receptor level ( Statements Author contributions JX: Writingoriginal draft, Writingreview and editing

For markers lacking physical position information, the coordinates of adjacent genetic markers were used to define the start and end points of the corresponding QTL region

Further studies are needed to determine the roles of altered iron homeostasis and ferroptosis in DMD