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Nonogaki, K., Nozue, K

In muscle injury model studies, muscle injury (such as trauma, exerciseinduced injury, or diseaserelated damage) triggers a series of pathophysiological processes including myofiber rupture, inflammatory responses, and muscle stem cell activation

Positive pressure inside the vial from repeated needle insertions can force contaminants back through the needle on subsequent draws, so always equalize pressure by injecting an equivalent volume of air before drawing liquid

A B12 supplement can also do the trick
