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RNA isolation and quantitative real-time PCR Total RNA was isolated by using TRIzol reagent (Invitrogen, Carlsbad, CA, USA)
doi: 10.1136/jcp.47.11.999 99 ChettyR
COVID-19-induced ARDS is associated with decreased frequency of activated memory/effector T cells expressing CD11a(+)
Occasionally imaging studies such as Xrays, ultrasound may also be performed if indicated
