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Evening administration faces three obstacles: circadian suppression of autophagy (PER2 gene expression peaks in the evening and actively inhibits autophagy initiation), postprandial insulin signaling (which blocks AMPK-mediated autophagy), and reduced lymphatic clearance during sedentary evening hours
At 10 M, growth was inhibited by 14.8%, and at 10 M by 81.5%
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