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After the bacterial layer had dried, 3 mL of EPA water (60 mg CaSO 4 2 H 2 O, 60 mg MgSO 4 , 4 mg KCl and 96 mg NaHCO 3 per liter of H 2 O) was added to form a thin liquid layer on the surface of the plates forming a medium known as biofilm, which we previously demonstrated to be the most suitable for these assays (Taipe Huisa et al