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5-Amino-1-methylquinolinium (5-Amino-1MQ) is a small-molecule inhibitor of nicotinamide N-methyltransferase (NNMT), the cytosolic enzyme that methylates nicotinamide to 1-methylnicotinamide (1-MNA) using S-adenosyl-L-methionine (SAM) as the methyl donor

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Researchers confirmed this through knockout studies in mice, where animals lacking beta-3 adrenergic receptors showed zero response to AOD 9604, proving the peptide works specifically through this receptor pathway in adipose tissue

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