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On the outer face of the inner mitochondrial membrane, glycerophosphate dehydrogenase complex (mitochondrial glycerophosphate dehydrogenase) oxidizes glycerophosphate back to dihydroxyacetone phosphate, a reaction not generating NADH but reducing a flavin prosthesic group

Primary antibodies were applied overnight at 4 C, followed by HRP-conjugated secondary antibodies for 1 h at room temperature

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