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The tissue was disrupted using tissue homogenizer Bio-Gen PRO200 homogenizer (PRO Scientific, Oxford, CT, USA), in 1000 l of TRIzol (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) and the isolation itself was done using a TRIzol-based reagent method according to the manufacturers instructions

There are no conversion costs for those who are prescribed oral supplements as an initial therapy, and so the savings could be even greater than reported