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RNA and lentivirus interference For the interference of ALDH3A1, ViewSolidBiotech (Beijing, China) designed and prepared two siRNA sequences (Si1: 5-GGAACUCAGUGGUCCUCAATT-3 and Si2: 5-UUGAGGACCACUGAGUUCCTT-3) and a negative control siRNA (siNC)

Gel filtration chromatography 5.1 For protein purification Shape and mass of proteins are unique

These mast cell mediators provoke potent inflammatory responses that can include urticaria (AKA hives-skin rash and swelling), angioedema (swelling beneath the skin surface), bronchoconstriction (airway constriction), diarrhea, vomiting, hypotension (low blood pressure), cardiovascular collapse, and death, all within a matter of minutes