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Anti-neurodegenerative benefits of acetylcholinesterase inhibitors in Alzheimers disease: Nexus of cholinergic and nerve growth factor dysfunction

In addition, a foreign in vitro experimental study on human glioma A172 cells found that metformin increased the expression of AMPK/pAMPK/Bax protein and decreased the expression of mTOR/Bcl-2 protein in a dose and time-dependent manner, thereby inhibiting the proliferation and apoptosis of A172 cells

doi: 10.4315/0362-028X.JFP-17-214

On the following day, blots were washed with TBST and incubated with the secondary fluorophore-conjugated antibody (LI-COR