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In the first-hit, the level of intrahepatic diacylglycerol (DAG) enhanced with the increase of saturated fat content, and the activity of mitochondrial respiratory chain complex in liver tissue decreased

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The phosphoinositide 3-kinase (PI3K) class of intracellular enzymes is involved in multiple cell signaling pathways, including the well-studied PI3K-Akt pathway, and is involved in various cellular functions, such as cell growth, proliferation, and differentiation [15]

This limitation matters because repeated-dose toxicity guidelines normally expect systematic clinical observations, body weight tracking, hematology, clinical chemistry, organ weights, and histopathological examination under a defined study design