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Nutritional Cofactors and Lifestyle Choices Avoid excess NSAIDs or alcohol Maintain magnesium, potassium, and vitamin B6 levels Move regularly to improve circulation Gut and Microbiome: The Unsung Detox Heroes The Gut-Liver Axis and Detox The gut and liver are connected via bile and blood

The pH of the medium was adjusted to 5.80 using NaOH/HCl (1 N), and 0.8% agar (SRL, Mumbai, India) was added prior to sterilization at 121 C (15 psi) for 20 min in an autoclave (York Scientific, Ghaziabad, India)

Following DNA damage, the cell cycle can be transiently arrested to allow for DNA repair or activation of pathways leading to programmed cell death (apoptosis) when the damage cannot be repaired (29)