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Description
The fish were trained to locate a specific sponge site that contained food rewards 28

Samples were then precipitated by ice cold H 2 O/MeOH/CHCl 3 (v/v 4:4:1) washed and resuspended in 50 mM HEPES buffer (pH 7.4)

Changes are subtle in the early days

It is a relatively safe drug even for long-term administration (Raut et al., 2012)
