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Some people, particularly those who were severely deficient, report a sudden improvement in energy, focus, or mood within 24 to 72 hours

4ad, genomic DNA was extracted according to Qiamp Fast DNA Stool extraction kit (Qiagen) and the V3V4 region of the bacterial 16S rRNA gene was amplified with the NEBNext Q5 Hot Start Hifi PCR Master Mix (NEB) using a dual-index strategy

Factors affecting results Individual variation is significant

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