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Malondialdehyde content was estimated using the chromophore detection method at 532 nm, as described by Buege and Aust (1978)

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Lubos E, Loscalzo J, Handy DE

With the rise of Arabidopsis as a study system, and notably genome sequencing, the pathway and its genes were characterized at the molecular genetic level, revealing (or confirming) that all four enzymes were encoded by more than one gene, thus explaining the presence of the enzyme activities in several compartments (Table 1)