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One microgram of total RNA was used for cDNA synthesis using the TransScript Reverse Transcription Kit (TransGen Biotech, Beijing)

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These are limited by side effects such as dry mouth, constipation, and blurred vision Botulinum toxin injections for localised hyperhidrosis (its use is approved for axillary hyperhidrosis) Iontophoresis, where an electric current is passed through skin soaked in water with or without an anticholinergic medication
