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For OCR determination, assays were initiated by replacing the media with XF assay media supplemented with fresh sodium pyruvate (1 mM), glutamine (2 mM), glucose (0.5 mg/L), and 10% FBS (pH 7.4), and were equilibrated in non-CO 2 incubator at 37 C for 1 h
Endocrinology 124 , 4048 (1989)
This process is mediated by a protein called glycine transporter 1, or GLYT1
Another direction may include screening for B12 deficiency within the practice
