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The liver tissue was homogenized with a buffer solution having 250 mM sucrose, 50 mM Tris-HCl (pH 7.4), and 1 mM EDTA and stored with 4% paraformaldehyde (PFA) or at 80C
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Redox Biology;2015 Jul J.W

Histological analysis revealed that 5 amino 1mq treatment reduced adipocyte size by 30-40%, indicating genuine fat loss rather than simple fluid shifts
