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For anaerobic growth, experiments were typically set up under ambient conditions and subsequently incubated statically at 37 C inside an anaerobic chamber (Coy Laboratory Products), except for the RNA-seq time course, RT-qPCR, and proteomics experiments, which were not only incubated anaerobically but also set up under anaerobic conditions given the relatively short experimental timeframe

doi: 10.1016/j.bbrep.2019.100618 556 MacRaeHSMefferdKM

If these antibodies are present, our stomach won't produce enough intrinsic factor or HCl (Hydrochloric Acid), leading to severe B12 deficiency

Thus, tissue concentrations are expected to be much higher than the IC 50 of 1.351.8 M that we measured against SARS-CoV-2