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To assess speciophylline in positive allosteric mode, cells were treated with test compounds over a concentration range of 10 4 to 5 10 9 M in the presence of 15 M forskolin for 15 min at 37 C, followed by the addition of the EC 20 concentration of the met-enkephalin and incubation for 90 min at 37 C

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Here's what's specific to DSIP: it's a small peptide with a notoriously short biological half-life about 15 minutes due to a specific aminopeptidase that cleaves it rapidly

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