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Fluorescent staining was visualized and images were acquired with a 63x oil immersion objective (NA = 1.2) with a 2x optical zoom on a Leica Stellaris 5 White Light Laser Confocal Microscope coupled to a camera integrated with LasX software for Lightning deconvolution (Leica Biosystems, Deer Park, IL, USA)

Inflammatory molecules and pathways in the pathogenesis of diabetic nephropathy

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