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All cell lines were cultured in RPMI 1640 media (Gibco) supplemented with 10% Fetal Bovine Serum (FBS) (Gibco), 1% L-Glutamine (Gibco) and 1% Penicillin/Streptomycin (Gibco)

PMID 16537422

Although it catalyzes the metabolism of methionine, homocysteine (Hcy) is an amino acid typically not involved in protein synthesis

However, the steroid injection requires just one session that combines diagnosis and treatment, where the RFA procedure typically requires 3-4 sessions initially
