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AOD-9604, CJC-1295, ipamorelin acetate, thymosin alpha-1, and Selank acetate were withdrawn from review

The retinal microenvironment is prone to oxidative damage due to high oxygen consumption [217], exposure to visible light (400700 nm), and the presence of high levels of polyunsaturated fatty acids and photosensitive molecules, including, lipofuscin and rhodopsin

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After that, the samples were washed three times in PBS-PVA and placed on slides in a 1 L drop of SlowFade with 5 g/mL Hoechst 33,342 under a coverslip