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The ThT kinetic curve is characterized by a lag time ( t lag , the time in which ThT fluorescence reaches 5% of its maximum), dominated by the formation of oligomeric species not detectable by ThT, followed by an elongation phase, in which pre-fibrillar structures elongate to fiber, normally characterized by a time to half ( t 1/2 , the time in which the ThT fluorescence reaches half of its maximum value) and, finally, a plateau region in which the equilibrium is reached and the ThT fluorescence reaches its maximum value I max (Figure S1 in the Supporting Information)

Probiotic Escherichia coli Nissle 1917 inhibits leaky gut by enhancing mucosal integrity

doi: 10.3389/fonc.2019.00024 391

Methods: A retrospective observational study was carried out in Lithuanian University of Health Sciences hospital - the largest teaching facility of tertiary care in country