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Mitochondrial footprint and mean branch length were determined from unfiltered binarized images using the MiNA (v2.0) FIJI plugin ( RNAseq Hypoxic cells were lysed in Buffer RLT without -mercaptoethanol (Qiagen, Germantown, MD, USA)

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Muscular Mitochondrial Function in Amyotrophic Lateral Sclerosis Is Progressively Altered as the Disease Develops: A Temporal Study in Man. Experimental Neurology 198: 2530