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Interestingly, this P R RA R insertion generates a furin cleavage site on S-protein at the S1/S2 multi-basic region, considered as a potential gain of function for the viral pathogen
Lysates were then precipitated using trichloroacetic acid/acetone and resuspended in 100 mM Tris-HCl, 6 M urea, 2 M thiourea, pH 8.0
