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The pH values of the samples were determined using a Metrohm 6.0204.100 combined pH glass electrode, calibrated by aqueous NBS standard buffer solutions
In men, MNP accumulation in the testes is accompanied by spermatogenesis disruption through pyruvate and thyroid metabolism imbalance [202], destabilization of the blood-testis barrier through ROS/MAPK/Nrf2/p38/NF-B activation [205], and suppression of the hypothalamic-pituitary-gonadal (HPG) axis through reduced testosterone and LHR/cAMP/PKA/StAR [52], clinically manifesting as oligozoospermia and asthenozoospermia, confirmed by the detection of PS, PE, and PVC in semen [207,208,209]
eds.), pp.187220
