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To test whether any of the genes in the downregulated putative phage locus might act as a repressor of biofilm formation, with that repression potentially being relieved by LCA, we also generated deletion mutants lacking different segments of this locus (leaving intact a predicted bacterial gene on the opposite strand in the middle of the locus, whose expression was unaffected by LCA)

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This is because cysteine is at the very beginning of the two-step cellular synthesis of glutathione (see Figure 1)

doi: 10.3390/nu11020389 90 TurnlundJ