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& Stocchi, V
3.5 Removal of GST affinity tag after protease cleavage in solution If the protein was digested in solution, the target protein can be purified further by re-chromatography on the glutathione Sepharose column to remove any un-cleaved fusion protein and the GST moiety
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Therefore, there is an urgent need for the scientific community to find effective ways to inhibit the proliferation and metastasis of CRC cells

According to manufacturer instructions for our assay kit, best practice is to deproteinize plasma prior to storage
