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It is also important to mention that RNA and protein levels were determined by real-time PCR and Western blotting, respectively

10.1016/j.scitotenv.2017.09.009 17 de HaanW.de Vries-van der WeijJ.MolI
A total of 399,396 boxes (640 pixels, 703 ) were extracted and downsampled to 512 pixels (1.3738 per pixel), then 2 binned for faster processing

The latter approach delays claims processing but ensures accurate code assignment aligned with CMS coding guidelines