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Description
Perfusion was performed by flushing pre-warmed RPMI containing phenol red and L-glutamine through a 25- and 3/8-gauge needle placed into the aorta attached to Tygon tubing aided by the Masterflex L/S perfusion pump as described 81
Dosage and Administration: For Parenteral Use Only after dilution or dissolution of compatible drugs, following manufacturer instructions: Do Not Use Directly : Not suitable for direct IV, IM, or SC injection without first being mixed with a solute
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Cells were washed with PBS and incubated with 1 mL JC-1 working solution at 37 C for 20 min
