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One min later, a liver portion was excised and homogenized at 4C using lysis buffer containing 50 mM NaF, 1% TritonX100 and 100 mM Tris-HCl, 5 mM sodium pyrophosphate, and 10 mM EDTA (pH 7.2) and supplemented with 1 mM sodium orthovanadate, 1 mM phenylmethylsulfonyl-fluoride (PMSF), 2 g/mL aprotinin, 2 g/mL pepstatin, and 2 g/mL leupeptin as antiproteases