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[DOI] [PubMed] [Google Scholar] 54

Unilamellar vesicles were obtained by sequential extrusion of the MLVs dispersions in an Avanti Mini-Extruder, through a polycarbonate membrane of 100 nm pore size at around ten degrees centigrade above the transition temperature of the lipid mixture in the appropriate proportion taking into account the literature transition temperature [14-18]

J Urol 1999;161:1980-3

For cloning purposes, cells were grown in lysogeny broth (LB) medium (10 g L 1 tryptone, 5 g L 1 yeast extract, and 10 g L 1 sodium chloride) at 37C on a shaking incubator (shaking frequency: 180 min 1 , eccentricity: 25 mm)