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The concentration of ferritin also decreased as the cycle progressed, and anemia was found to be at its highest in the middle of the cycle

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The samples were subjected to click reaction by adding 1 l rhodamine azide (10 mM in DMSO), 5 l BTTAA (10 mM in DMSO), 2 l CuSO 4 (50 mM in ddH 2 O) and 2 l NaAsc (100 mM in ddH 2 O) to 90 l of each sample and incubated for 1 h at r.t
