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Kaori Otsuki (RIKEN CBS RRD) for providing equipment and technical assistance with the microdialysis experiments

Immunohistochemistry (IHC) staining was performed with a universal detection kit (PV-6000, ZSGB-Bio, Beijing, China) and DAB Detection System (ZLI-9017, ZSGB-Bio, Beijing, China)

Taking glutathione 12 hours before bed ensures your liver has adequate glutathione precisely during this overnight repair window

There is also a possibility of central adaptation: with repeated or chronic VNS, the brainstem might adjust to the continuous afferent input, potentially raising the threshold for response over time (Vadhan and Tadi 2019)