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ATPase activity assays confirmed that coumarin stimulates ATP hydrolysis by P-glycoprotein (P-gp), indicating that coumarin acts as a substrate for it, and the nanoparticles were shown to inhibit P-gp-mediated efflux, enhancing drug retention in cells

As RNA-seq results for PreNAC specimens have been previously reported, this study performed a comprehensive gene expression analysis using total RNA extracted from PostNAC FFPE surgical specimens [12]